Selectivity of a bromoacridine-containing fluorophore for triplex DNA

Abstract Fluorophore 1,8-naphthilamide was linked to 2-bromoacridine through an ethylenediamine spacer using a succinct synthetic route give bromoacridine-linked, bifunctional fluorophore conjugate for the detection of triplex DNA. Acridine is well known intercalate into duplex DNA whereas introduction bulky bromine atom at position C2 redirects specificity over In this work, photoelectron transfer assay used demonstrate that synthesised 2-bromoacridine-linked had good selectivity representative target sequence d(T*A.T) 20 compared with double-stranded d(T.A) , single-stranded dT or d(G/A) 19 sequences. Graphic abstract